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Registro Completo |
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
21/12/2016 |
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Data da última atualização: |
21/12/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TIAGO, A. V.; ROSSI, A. A. B.; TIAGO, P. V.; CARPEJANI, A. A.; SILVA, B. M.; HOOGERHEIDE, E. S. S.; YAMASHITA, O. M. |
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Afiliação: |
A. V. TIAGO, UNEMAT-ALTA FLORESTA; A. A. B. ROSSI, UNEMAT-ALTA FLORESTA; P. V. TIAGO, UNEMAT-ALTA FLORESTA; A. A. CARPEJANI, UNEMAT-ALTA FLORESTA; B. M. SILVA, UNEMAT-ALTA FLORESTA; EULALIA SOLER SOBREIRA HOOGERHEIDE, CPAMT; O. M. YAMASHITA, UNEMAT-ALTA FLORESTA. |
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Título: |
Genetic diversity in cassava landraces grown on farms in Alta Floresta-MT, Brazil |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Genetics and Molecular Research, vol. 15, n. 3, set. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Brazil is considered one of the domestication centers of cassava (Manihot esculenta), containing a large part of the biological diversity and traditional knowledge of the species. The aim of the present study was to evaluate the genetic diversity of cassava landraces grown by farmers in the north of Mato Grosso State, Brazil, using inter simple sequence repeat (ISSR) molecular markers. The study was carried out in the municipality of Alta Floresta, MT, on farms located in two rural areas. Seventeen cassava landraces were selected. The DNA was extracted and polymerase chain reaction amplifications were performed using 15 ISSR primers. Genetic similarity estimates were calculated using Jaccard?s index and the generated matrix was used for clustering the genotypes by using UPGMA and Tocher?s methods. The 15 ISSR primers amplified 120 fragments, revealing 61.67% polymorphism. The polymorphism information content ranged from 0.04 to 0.61, averaging 0.39. The most similar genotypes were AF5 and AF8, whereas the least similar were AF1 and AF16. The UPGMA clustering method formed five groups. Group I included twelve landraces, Group II contained two, and the other groups contained one landrace each. Tocher?s method resulted in six groups: 12 landraces clustered in one group, and the other groups each contained one landrace. The ISSR markers proved efficient in revealing genetic diversity among the cassava landraces. The landraces grown by farmers in the two rural areas of Alta Floresta have a great variability and, thus, can be exploited in programs for breeding and preservation of the species. MenosBrazil is considered one of the domestication centers of cassava (Manihot esculenta), containing a large part of the biological diversity and traditional knowledge of the species. The aim of the present study was to evaluate the genetic diversity of cassava landraces grown by farmers in the north of Mato Grosso State, Brazil, using inter simple sequence repeat (ISSR) molecular markers. The study was carried out in the municipality of Alta Floresta, MT, on farms located in two rural areas. Seventeen cassava landraces were selected. The DNA was extracted and polymerase chain reaction amplifications were performed using 15 ISSR primers. Genetic similarity estimates were calculated using Jaccard?s index and the generated matrix was used for clustering the genotypes by using UPGMA and Tocher?s methods. The 15 ISSR primers amplified 120 fragments, revealing 61.67% polymorphism. The polymorphism information content ranged from 0.04 to 0.61, averaging 0.39. The most similar genotypes were AF5 and AF8, whereas the least similar were AF1 and AF16. The UPGMA clustering method formed five groups. Group I included twelve landraces, Group II contained two, and the other groups contained one landrace each. Tocher?s method resulted in six groups: 12 landraces clustered in one group, and the other groups each contained one landrace. The ISSR markers proved efficient in revealing genetic diversity among the cassava landraces. The landraces grown by farmers in the two rural areas of Alta Flore... Mostrar Tudo |
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Palavras-Chave: |
Germplasm bank; Issr. |
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Thesagro: |
Manihot Esculenta. |
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Thesaurus Nal: |
Genetic resources; Microsatellite repeats. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/152174/1/2016-cpamt-hoogerheide-genetic-diversity-cassava.pdf
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Marc: |
LEADER 02351naa a2200253 a 4500
001 2059082
005 2016-12-21
008 2016 bl uuuu u00u1 u #d
100 1 $aTIAGO, A. V.
245 $aGenetic diversity in cassava landraces grown on farms in Alta Floresta-MT, Brazil$h[electronic resource]
260 $c2016
520 $aBrazil is considered one of the domestication centers of cassava (Manihot esculenta), containing a large part of the biological diversity and traditional knowledge of the species. The aim of the present study was to evaluate the genetic diversity of cassava landraces grown by farmers in the north of Mato Grosso State, Brazil, using inter simple sequence repeat (ISSR) molecular markers. The study was carried out in the municipality of Alta Floresta, MT, on farms located in two rural areas. Seventeen cassava landraces were selected. The DNA was extracted and polymerase chain reaction amplifications were performed using 15 ISSR primers. Genetic similarity estimates were calculated using Jaccard?s index and the generated matrix was used for clustering the genotypes by using UPGMA and Tocher?s methods. The 15 ISSR primers amplified 120 fragments, revealing 61.67% polymorphism. The polymorphism information content ranged from 0.04 to 0.61, averaging 0.39. The most similar genotypes were AF5 and AF8, whereas the least similar were AF1 and AF16. The UPGMA clustering method formed five groups. Group I included twelve landraces, Group II contained two, and the other groups contained one landrace each. Tocher?s method resulted in six groups: 12 landraces clustered in one group, and the other groups each contained one landrace. The ISSR markers proved efficient in revealing genetic diversity among the cassava landraces. The landraces grown by farmers in the two rural areas of Alta Floresta have a great variability and, thus, can be exploited in programs for breeding and preservation of the species.
650 $aGenetic resources
650 $aMicrosatellite repeats
650 $aManihot Esculenta
653 $aGermplasm bank
653 $aIssr
700 1 $aROSSI, A. A. B.
700 1 $aTIAGO, P. V.
700 1 $aCARPEJANI, A. A.
700 1 $aSILVA, B. M.
700 1 $aHOOGERHEIDE, E. S. S.
700 1 $aYAMASHITA, O. M.
773 $tGenetics and Molecular Research, vol. 15$gn. 3, set. 2016.
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Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
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| 5. |  | ALMEIDA, M. C. de; MONTEIRO, E. de C.; MARTINS, M. dos R.; ALVES, B. J. R.; BODDEY, R. M.; URQUIAGA, S. Avaliação do potencial produtivo e fixação biológica de nitrogênio em diferentes variedades de cana-de-açúcar como suporte para produção sustentável In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 20., 2020, Seropédica. Inteligência artificial: a nova fronteira da ciência brasileira: impactos para a agricultura sustentável. Resumos... Seropédica: Embrapa Agrobiologia, 2020.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 6. | | OLIVEIRA, C. A. G. de; SILVA, E. P. da; GUARESCHI, R. F.; MONTEIRO, E. de C.; ALMEIDA, M. C. de; ALVES, B. J. R.; URQUIAGA, S.; BODDEY, R. M. Potencial produtivo de três variedades de cana-de-açúcar em Argissolo com baixa disponibilidade de N e sem adubação nitrogenada. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 20., 2020, Seropédica. Inteligência artificial: a nova fronteira da ciência brasileira: impactos para a agricultura sustentável. Resumos... Seropédica: Embrapa Agrobiologia, 2020.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 7. | | ARAÚJO, F. R.; MADRUGA, C. R.; RAMOS, C. A. do N.; MELO, E. S. de P.; ALMEIDA, M. C. de; FRAGOSO, S. P.; KESSLER, R. H.; SOARES, C. O.; OLIVEIRA, M. B.; ONSELEN, J. van. Imunização de bovinos com MSP1a e MSP2 recombinantes de Anaplasma marginale com CpG ODN 2006 como adjuvante, e desafio com isolado heterólogo. In: SÉRIES Embrapa: [coletânea de publicações seriadas da Embrapa Gado de Corte - 2006 - 2007 -2008]. Campo Grande, MS: Embrapa Gado de Corte, 2009. 27 p. 1 CD-ROM. (Embrapa Gado de Corte. Boletim de pesquisa e desenvolvimento, 19)| Biblioteca(s): Embrapa Gado de Corte. |
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